67-64-1. If you deal with solvents or excipients on a regular basis then you know this is the CAS number for acetone.
What type of solvent do you use for your manufacturing processes? Depending on your application needs, did you know that one type of solvent is more efficient (and therefore saves more on costs) than others?
INFORMATION TO HELP YOU DECIDE
Acetone or acetonitrile? It will depend on what peptides or proteins you’re assaying for but your choice in solvent is important for everything from in vivo drug screening and bioaffinity screening to metabolite identification and glycoprotein mapping.
Fritz et al at the University of Rostock conducted a number of assays that found acetone is an overall better solvent than acetonitrile and methanol. Using liquid chromatography (LC) and mass spectrometry systems, the German based group compared five tryptic protein digests with either acetone, acetonitrile, or methanol as the solvent.
Here’s how acetone fared when compared with acetonitrile and methanol:
- Shorter overall analysis time due to lower peptide retention in LC
- Higher desolvation efficiency for peptides
- Lower toxicity levels based on LD50 (oral, rat; dermal methods)
This equates to cost-savings for your needs. It improves overall throughput time for your protocols, higher output efficiency, and may even extend the life of your LC column thanks to the lower viscosity of acetone that affects backpressure.